PNS-3D Services

Predictive PNS Models for Safety, Efficacy & Disease Insights

PNS-3D Organoid Services provide clinically relevant, human-derived models for peripheral neuropathy, offering functional and morphological insights through electrode-embedded plates. Standard and custom services support neuroprotection, safety, and disease modeling—bridging the gap between preclinical data and human outcomes to reduce neurological drug failure rates.

Safety Assessment

The peripheral nervous system is especially vulnerable to off-target damage from chemotherapeutics and other agents. PNS-3D technology enables early identification of neurotoxic potential by leveraging functional, clinically predictive electrophysiological metrics.

Unlike traditional safety assessments that rely on morphology or cell viability, PNS-3D delivers three-dimensional electrophysiological data that more accurately reflects nerve health and helps reduce the risk of peripheral neuropathy in clinical trials.

The chemotherapeutic vincristine triggers a rapid decline of nerve conduction in PNS-3D Organoids while vehicle-treated controls maintain robust function over time.

Efficacy Evaluation

PNS-3D technology enables precise evaluation of therapeutics designed to target the peripheral nervous system, supporting applications in neuroprotection, disease modeling, and neurorehabilitation. Its sensitive functional assays detect early recovery or preservation of electrophysiological function, providing a rigorous assessment of therapeutic efficacy for conditions like peripheral neuropathy.

Unlike traditional methods, which rely on delayed morphological changes, PNS-3D offers early, clinically relevant insights by capturing functional improvements before structural alterations appear.

Electrophysiological time-courses show that two of three SARM1 inhibitors (black traces) markedly delay vincristine-induced degeneration, preserving activity nearer the normalized vehicle baseline (blue) than vincristine alone (red). Green shading highlights intervals where inhibitor responses remain above the vincristine curve, providing evidence of neuroprotection, whereas red shading denotes periods of unmitigated functional loss.

Endpoints

Electrophysiology

Velocity Density Index (VDI)
Integrates nerve conduction velocity, response amplitude, and number of compound action potentials into one clinically predictive metric.

Stimulus Threshold
Determines nerve responsiveness sensitivity to electrical stimulation to characterize hyper- or hypo-excitability.

Nerve Conduction Velocity (NCV)
Evaluates nerve health through compound action potential transmission speed.

Response Amplitude
Indicates nerve signal strength and integrity through measurement of compound action potential amplitude.

Morphology & Omics

Nerve Fiber Length
Measurement of nerve fiber length distinguishing healthy and degenerating axonal structures.

Degeneration Index
Quantification of small particles generated as nerves fragment due to chemical or other insult.

Organoid Size & Roundness Analysis
Ensures consistency and reproducibility between replicates.

Downstream Sample Collection
Enables mechanistic omics studies (RNA sequencing, proteomics) and/or compound stability investigations (mass spec, etc).

Reporting

Upon completion, a comprehensive data package including processed, normalized functional and morphological endpoints, IC50/EC50 analyses, statistical significance, and representative imagery is delivered. Raw datasets can be provided for deeper analyses upon request.

Standard Services

PNS-3D Organoid Standard Services provide a physiologically relevant preclinical 3D nerve model combining human derived sensory neurons and Schwann cells to study peripheral neuropathy, neuroprotection, and electrophysiological function.

Custom Services

PNS-3D Organoid Custom Services offer tailored 3D nerve models with the ability to incorporate motor neurons, astrocytes, and disease-specific modeling for advanced studies in peripheral neuropathy, neuroprotection, and nerve regeneration.

Test Articles

Unlimited

Dose Response

5 or greater

Timepoint

2-5

Endpoints

Nerve conduction only
Nerve conduction
Nerve degeneration

Predictive PNS Models for Safety, Efficacy & Disease Insights

Safety Assessment

Efficacy Evaluation

Endpoints

Reporting

PNS-3D Organoid Services

PNS-3D Services Resources