CNS-3D Services

CNS Safety and Efficacy—Powered by CNS-3D Organoids

CNS-3D Organoid Services offer high-throughput, human-relevant assessments of neurotoxicity, efficacy, and inflammation—supporting precise, early-stage CNS drug development with customizable models and integrated functional and viability readouts

Central Nervous System

CNS-3D Organoid Standard Services

CNS-3D Organoid Standard Services are utilized for neurotoxicity, safety and efficacy assessments.

FLIPR Assessment

Spontaneous activity of CNS-3D Organoids enables reproducible, modulable assessment of neurotoxicity and cytotoxicity using FLIPR, requiring as few as four replicates per condition.

Morphology Analysis

Multiplexed assays enable detailed analysis of neuron and astrocyte morphology via 3D immunostaining, with optional media sampling for plate reader assays using as little as 5–25 µL

High-throughput Format

High-throughput assay format enables screening of up to 20,000 compounds per week using 384-well plates, with 12 compounds per plate tested in quadruplicate across a 7-point dose range.

Use Case 01

CNS Neurotoxicity Assessment

A study published in ALTEX found that CNS-3D Organoids were 7.41 times more accurate than traditional animal models at predicting clinical neurotoxicity because they contain multiple iPSC-derived neural cell types that capture the complexity of human brain tissue. Unlike basic cell cultures, CNS-3D Organoids measure functional electrical activity, enabling detection of early changes in neural network behavior that may indicate toxicity before structural damage occurs.

The standard assay design delivers human-predictive neurotoxicity score with >90% specificity and >50% sensitivity1, providing early diagnostic value and a fully-automated workflow.

Dose response data of 8 representative compounds with various degrees of toxicity as assessed by potency of neuromodulation (peak count) relative to clinically relevant concentrations (Cmax).

Use Case 02

Neuromodulation Assessment

Cortical organoids exhibiting spontaneous network-level activity enable comprehensive pharmacological profiling, including potency rank-ordering and on/off-target assessments. A proprietary waveform analysis package extracts more than 60 features from these organoids, generating rich, high-dimensional datasets that are well-suited for machine learning applications and phenotypic stratification.

Representative waveforms showing a variety of neuromodulation phenotypes.

Use Case 03

Neuroinflammation Assessment

Integrated functional network activity measured via FLIPR, combined with cell-type-specific high-content imaging and media supernatant analysis for cytokine profiling, enables a comprehensive, multi-scale view of neuroinflammatory responses. This approach facilitates the detection of compounds that trigger harmful inflammatory processes, supporting discovery efforts focused on identifying interventions that can prevent or reverse these detrimental phenotypes.

Representative images of GFAP staining (astrocyte) in CNS-3D organoids, before and after treatment with a neuroinflammatory insult.

CNS-3D Organoid Custom Services

Central Nervous System

Non-standard 3D Neural Models

Cell types include: microglia for neuroinflammation studies and oligodendrocytes for remyelination and demyelination disorder research.

A Variety of Models

Ability to generate induced, genetic or custom disease models utilizing CNS-3D Organoids.

Reporting

At the end of each study a detailed report with fully analyzed graphs, IC/EC50 values and representative images will be compiled. Links to the raw data will be provided in addition to a scheduled meeting to review the data.

Report will highlight which compounds have potential neurotoxicity issues.