This application note summarizes key findings from the 28bio Endurance Study, highlighting how CNS-3D Brain Organoids, combined with an AI neurotoxicology prediction model, enable a highly accurate assessment of clinical seizure risk early in drug development.

‍

Human clinical seizure prediction

AI neurotoxicology prediction model integrating functional and chemical features achieved 83% sensitivity and 89% specificity, enabling confident seizure risk prediction while minimizing false positives.

‍

Physiologically relevant and functional

Human iPSC-derived CNS-3D Brain Organoids exhibit spontaneous calcium oscillations for functional assessment of neuronal activity.

‍

Actionable early-stage screening

CNS-3D supports earlier, higher confidence neurotoxicity risk assessment than conventional nonclinical models.