Application Note
Chemotherapy-Induced Peripheral Neuropathy Assessment in PNS-3D Technology

PNS-3D technology provides a reproducible and physiologically relevant model of peripheral neuropathy, integrating longitudinal electrophysiology, morphological assessments, and transcriptomic profiling. Using vincristine as a benchmark chemotherapeutic, the technology captures dose- and time-dependent functional decline, structural degeneration, and sensory neuron-specific transcriptomic changes. Its high sensitivity supports tracking neuropathy progression via IC50 analysis across multiple time points.

The technology enables evaluation of diverse chemotherapeutics and antibody-drug conjugates, revealing distinct neurotoxicity profiles across functional and morphological endpoints. These analyses differentiate compounds that cause early functional impairment without structural damage from those exhibiting closely aligned neurotoxic effects.

SARM1 inhibition studies further demonstrate partial functional neuroprotection and preserved morphology during CIPN, underscoring the platform’s utility for therapeutic modulation. PNS-3D recapitulates key aspects of human CIPN pathophysiology, supports mechanistic differentiation of neurotoxicity, and facilitates evaluation of neuroprotective strategies.