This application note demonstrates how CNS-3D Brain Organoids can be applied for the accurate prediction of ASO neurotoxicity, supporting confident prioritization of ASO candidates prior to in vivo studies.

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Accurate ASO neurotoxicity prediction

CNS-3D achieved 92% sensitivity and 92% specificity across a panel of 24 ASOs, enabling confident identification of neurotoxic liabilities while minimizing false positives.

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Functional, high-throughput assessment

Human iPSC-derived CNS-3D Brain Organoids exhibit spontaneous calcium oscillations for functional assessment of neuronal activity.

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Actionable early-stage screening

CNS-3D supports earlier, data-driven prioritization of ASOs based on functional neurotoxicity risk ahead of in vivo studies.