CNS-3D Organoids are a high-throughput in vitro human model for investigating neurotoxicity, seizure liability, synaptogenesis, neurogenesis, excitotoxicity, ion channel activity, and neuropharmacology. These brain organoids support multiplexed, high-content analysis across diverse therapeutic modalities, including small molecules, siRNAs, antisense oligonucleotides (ASOs), adeno-associated viruses (AAVs), and antibody-based therapeutics.